Coding

Part:BBa_K4335013:Design

Designed by: Hang Zhang   Group: iGEM22_UESTC-BioTech   (2022-10-10)


SpCas9


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 241
    Illegal PstI site found at 787
    Illegal PstI site found at 2209
    Illegal PstI site found at 2413
    Illegal PstI site found at 2443
    Illegal PstI site found at 3655
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 241
    Illegal PstI site found at 787
    Illegal PstI site found at 2209
    Illegal PstI site found at 2413
    Illegal PstI site found at 2443
    Illegal PstI site found at 3655
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
    Illegal BglII site found at 1043
    Illegal BglII site found at 1733
    Illegal BglII site found at 3812
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 241
    Illegal PstI site found at 787
    Illegal PstI site found at 2209
    Illegal PstI site found at 2413
    Illegal PstI site found at 2443
    Illegal PstI site found at 3655
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 241
    Illegal PstI site found at 787
    Illegal PstI site found at 2209
    Illegal PstI site found at 2413
    Illegal PstI site found at 2443
    Illegal PstI site found at 3655
    Illegal NgoMIV site found at 1075
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3468


Design Notes

Source

Streptococcus pyogenes

References